Journal:
Article Title: Autotaxin signaling via lysophosphatidic acid receptors contributes to vascular endothelial growth factor-induced endothelial cell migration
doi: 10.1158/1541-7786.MCR-09-0288
Figure Lengend Snippet: A. qRT-PCR was used to measure Akt1, Akt2 and Akt3 mRNA levels in ATXMO-treated HUVEC. Next, HUVEC were treated with indicated concentrations of LPA for 16 h, then qRT-PCR was performed to measure: B. Akt1 and Akt3 expression or C. Akt2 expression. Finally, treatment of HUVEC with LPA1-specific SiRNA resulted in: D. >10-fold decrease in LPA1 expression and E. changes in Akt1, Akt2 and Akt3 mRNA levels. Statistical analysis for (A, D, E) utilized unpaired two-tailed t-tests and for (B, C) utilized one-way ANOVA with Dunnett's post test. In all cases, results, compared to control (MisMO in A, untreated in B & C, scrambled siRNA in D & E), are shown as: * (P < 0.05), ** (P < 0.01), or *** (P < 0.001).
Article Snippet: LPA1 gene silencing in HUVEC SiRNA targets for LPA1 were identified using siRNA Target Finder algorithm (Applied Biosystems) and constructed with the Silencer siRNA Construction Kit using directions supplied by the manufacturer (Applied Biosystems).
Techniques: Quantitative RT-PCR, Expressing, Two Tailed Test